Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Cell lysate - whole cell (Gastrocnemius)
Specification
Gastrocnemius
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey + 0.2% tween
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/15000
Detection
Detection method
Infrared Odyssey
Bands
Specific: 47 kDa
Exposure
4 minute(s) and 0 second(s)
Additional data
Additional Notes
Strong band detected in this optimisation experiment, it is recommended to upload less protein e.g. 30-40 ug
1= Marker Lane
2= Mouse Gastrocnemius
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
投稿 Apr 11 2014