Anti-Aspartate Aminotransferase 抗体 (ab221939)
Key features and details
- Rabbit polyclonal to Aspartate Aminotransferase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Aspartate Aminotransferase antibody
Aspartate Aminotransferase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Aspartate Aminotransferase -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Orangutan -
免疫原
Recombinant fragment corresponding to Human Aspartate Aminotransferase aa 350 to the C-terminus.
Database link: P17174 -
ポジティブ・コントロール
- ICC/IF: U-2 OS cells. IHC-P: Human liver tissue. WB: Human liver and tonsil lysates; RT4 and U-251 MG cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a key role in amino acid metabolism. -
配列類似性
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 450664 Chimpanzee
- Entrez Gene: 281206 Cow
- Entrez Gene: 101865666 Cynomolgus monkey
- Entrez Gene: 100060793 Horse
- Entrez Gene: 2805 Human
- Entrez Gene: 14718 Mouse
- Entrez Gene: 100173864 Orangutan
- Entrez Gene: 396967 Pig
see all -
別名
- AATC_HUMAN antibody
- Aspartate aminotransferase 1 antibody
- Aspartate aminotransferase antibody
see all
画像
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All lanes : Anti-Aspartate Aminotransferase antibody (ab221939) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 46 kDa -
Paraffin-embedded human liver tissue stained for Aspartate Aminotransferase using ab221939 at 1/1000 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Aspartate Aminotransferase (green) using ab221939 at 4 µg/ml in ICC/IF.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab221939 は 1 報の論文で使用されています。
- Wei XB et al. Exosome-Derived lncRNA NEAT1 Exacerbates Sepsis-Associated Encephalopathy by Promoting Ferroptosis Through Regulating miR-9-5p/TFRC and GOT1 Axis. Mol Neurobiol 59:1954-1969 (2022). PubMed: 35038133