Anti-Argininosuccinate Lyase 抗体 (ab96215)
Key features and details
- Rabbit polyclonal to Argininosuccinate Lyase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Argininosuccinate Lyase antibody
Argininosuccinate Lyase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Argininosuccinate Lyase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow -
免疫原
Recombinant fragment corresponding to Human Argininosuccinate Lyase aa 86-335.
Database link: P04424 -
ポジティブ・コントロール
- WB: MOLT-4, HeLa, IHC-P: DLD1 xenograft. ICC/IF: HeLa.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96215の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1. -
関連疾患
Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. -
配列類似性
Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. -
翻訳後修飾
Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. - Information by UniProt
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参照データベース
- Entrez Gene: 435 Human
- Entrez Gene: 109900 Mouse
- Omim: 608310 Human
- SwissProt: P04424 Human
- SwissProt: Q91YI0 Mouse
- Unigene: 632015 Human
- Unigene: 23869 Mouse
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別名
- Argininosuccinase antibody
- Argininosuccinate lyase antibody
- Arginosuccinase antibody
see all
画像
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Paraffin embedded DLD1 xenograft stained for ASL using ab96215 at 1/500 dilution in immunohistochemical analysis.
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HeLa cells stained for ASL (green) using ab96215 at 1/500 dilution in ICC/IF.
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All lanes : Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell extract
Lane 2 : Argininosuccinate Lyase Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa -
Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab96215 は論文での使用が確認できていません。