Anti-Angiopoietin-like 4/ANGPTL4 抗体 (ab115798)
Key features and details
- Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Angiopoietin-like 4/ANGPTL4 antibody
Angiopoietin-like 4/ANGPTL4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Angiopoietin-like 4/ANGPTL4 (internal sequence).
(Peptide available asab123817) -
ポジティブ・コントロール
- Breast cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115798の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
The detection limit for Angiopoietin-like 4/ANGPTL4 is approximately 2.5ng/lane under non-reducing and reducing conditions. |
特記事項 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 45 kDa. The detection limit for Angiopoietin-like 4/ANGPTL4 is approximately 2.5ng/lane under non-reducing and reducing conditions. |
ターゲット情報
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機能
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
組織特異性
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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参照データベース
- Entrez Gene: 51129 Human
- Omim: 605910 Human
- SwissProt: Q9BY76 Human
- Unigene: 9613 Human
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別名
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
see all
画像
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All lanes : Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab115798)
Lane 1 : Human Placenta Tissue Lysate at 50 µg
Lane 2 : SMMC Whole Cell Lysate at 40 µg
Lane 3 : HeLa Whole Cell Lysate at 40 µg
Predicted band size: 45 kDa -
All lanes : Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab115798)
Lane 1 : Smmc Whole Cell Lysate
Lane 2 : A549 Whole Cell Lysate
Lane 3 : U87 Whole Cell Lysate
Lane 4 : 293T Whole Cell Lysate
Lane 5 : SGC Whole Cell Lysate
Predicted band size: 45 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab115798 は 5 報の論文で使用されています。
- Cai YC et al. ANGPTL4 overexpression inhibits tumor cell adhesion and migration and predicts favorable prognosis of triple-negative breast cancer. BMC Cancer 20:878 (2020). PubMed: 32928141
- Kamermans A et al. Reduced Angiopoietin-Like 4 Expression in Multiple Sclerosis Lesions Facilitates Lipid Uptake by Phagocytes via Modulation of Lipoprotein-Lipase Activity. Front Immunol 10:950 (2019). PubMed: 31130950
- Chellan B et al. Enzyme-modified non-oxidized LDL (ELDL) induces human coronary artery smooth muscle cell transformation to a migratory and osteoblast-like phenotype. Sci Rep 8:11954 (2018). PubMed: 30097618
- Li B et al. TGF-ß2-induced ANGPTL4 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone. Oncotarget 8:54966-54977 (2017). PubMed: 28903395
- Florea V et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013). WB . PubMed: 24039874