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Synthetic peptide corresponding to Human Alpha-synuclein aa 116-131. Synthetic peptide of human synuclein (116-131) coupled to diphtheria toxoid with cysteine residue attached to N-ter of peptide. Conjugate cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).
Our Abpromise guarantee covers the use of ab6162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use a concentration of 2 µg/ml.|
|Dot blot||Use a concentration of 0.5 µg/ml.|
|IHC-Fr||1/500 - 1/1000.|
|IHC-P||Use a concentration of 1 µg/ml. PubMed: 20106867|
|WB||Use a concentration of 0.5 µg/ml.|
IHC-FoFr image of Alpha Synuclein staining on alpha Synuclein Trasgenic mouse brain section stained using ab6162. The animal were perfuse with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton 0.3X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody ab 6162, 2 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 anti-sheep IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab6162 staining Alpha-synuclein in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with PB ab64226 for 10 minutes at room temperature and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) for 30 minutes. An undiluted HRP-conjugated rabbit anti-goat polyclonal was used as the secondary antibody.
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