Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Permeabilization
Yes - Methanol
Specification
Brain sections
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Acetone
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Anti Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
fixation by absolute acetone permeabilization by absolute methanol ,blocking by 5% BSA in PBS
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投稿 Sep 16 2013