Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
10 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: odyssey blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: odyssey Secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
Licor Odyssey
Exposure
1 second(s)
Bands
Specific: 16 kDa
Positive control
Lane one: Wild-type mouse brain
Negative control
Lane two: Alpha synuclein Knockout mouse brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 16 2013