Application
Western blot
Sample
Human Cell lysate - whole cell (adipocytes)
Loading amount
5 µg
Specification
adipocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL
Exposure
40 second(s)
Bands
Specific: 20 kDa Non-specific: 35-55 kDa
Positive control
two different donors of adipocytes shown
Additional data
Additional Notes
molecular marker from bottom to top 25, 32, 47, 62, 83, 175 kD
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 10 2010