HRP Anti-Aldolase 抗体 (ab181662)
Key features and details
- HRP Goat polyclonal to Aldolase
- Suitable for: IP, WB
- Reacts with: Rabbit, Human
- Conjugation: HRP
- Isotype: IgG
製品の概要
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製品名
HRP Anti-Aldolase antibody
Aldolase 一次抗体 製品一覧 -
製品の詳細
HRP Goat polyclonal to Aldolase -
由来種
Goat -
標識
HRP -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Rabbit, Human
交差が予測される動物種: Mouse, Rat -
免疫原
Full length native protein (purified) corresponding to Rabbit Aldolase aa 1-364. Purified from Rabbit Muscle.
Sequence:MPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTE NTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKS KGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRC VLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLK RCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKYSHEEIA MATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTF SYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAG AAASESLFIS NHAY
Database link: P00883 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate, 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181662の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
1/100.
|
|
WB |
1/500 - 1/5000. Predicted molecular weight: 39 kDa.
|
特記事項 |
---|
IP
1/100. |
WB
1/500 - 1/5000. Predicted molecular weight: 39 kDa. |
ターゲット情報
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機能
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. -
パスウェイ
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
関連疾患
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. -
配列類似性
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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参照データベース
- Entrez Gene: 226 Human
- Entrez Gene: 11674 Mouse
- Entrez Gene: 100009055 Rabbit
- Entrez Gene: 24189 Rat
- Omim: 103850 Human
- SwissProt: P04075 Human
- SwissProt: P05064 Mouse
- SwissProt: P00883 Rabbit
see all -
別名
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
see all
画像
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HRP Anti-Aldolase antibody (ab181662) at 1/1000 dilution + A293 whole cell lysate
Developed using the ECL technique.
Predicted band size: 39 kDa4-12% tris glycine gradient gel for SDS-PAGE
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Immunoprecipitation and Western Blot analysis.
300 µl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are:
1. Purified aldolase
2. 300 µl antiserum with no antigen (negative control)
3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml)
4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml)
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Immunoprecipitation was performed with 300 μl of anti-Aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS. Rabbit Adolase (from rabbit muscle) was used.
1. Purified Aldolase (1μg)
2. Precipitation negative control (antiserum + No antigen)
3. 300 μl antiserum + 20 μl Aldolase
4. 300 μl antiserum + 80 μl Aldolase
5. 300 μl antiserum + 120 μl Aldolase
6. 300 μl antiserum + 150 μl Aldolase
7. 300 μl antiserum + 300 μl Aldolase
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab181662 は 5 報の論文で使用されています。
- Xu Y et al. MCM4 in human hepatocellular carcinoma: a potent prognostic factor associated with cell proliferation. Biosci Trends 15:100-106 (2021). PubMed: 33716256
- Gao Y et al. Propofol mediates pancreatic cancer cell activity through the repression of ADAM8 via SP1. Oncol Rep 46:N/A (2021). PubMed: 34617574
- Jia Q et al. Effects of GPR81 silencing combined with cisplatin stimulation on biological function in hypopharyngeal squamous cell carcinoma. Mol Med Rep 22:1727-1736 (2020). PubMed: 32582969
- Zhang W et al. Effects of Vitamin A on Expressions of Apoptosis Genes Bax and Bcl-2 in Epithelial Cells of Corneal Tissues Induced by Benzalkonium Chloride in Mice with Dry Eye. Med Sci Monit 25:4583-4589 (2019). PubMed: 31257361
- Song L et al. Exploring the active mechanism of berberine against HCC by systematic pharmacology and experimental validation. Mol Med Rep 20:4654-4664 (2019). PubMed: 31545468