Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
35 µg
Specification
U2OS
Treatment
infected with Adenovirus 5
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/10000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: gt anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Detection
Detection method
odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 100, 98, 65, 60, 50, kDa
Positive control
U2OS cells infected with wt Adenovirus5
Negative control
Uninfected U2OS cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 27 2011