Application
Western blot
Sample
Human Cell lysate - whole cell (A293 Cells)
Loading amount
300 µg
Specification
A293 Cells
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in pbs with 0.5% tween
Secondary antibody
Name
Non-Abcam antibody was used: IRDYE 680 donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Dilution
1/5000
Detection
Detection method
Licor Odyssey
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 25, 64, 108 kDa
Positive control
A293 cells that were not treated with the virus.
Additional data
Additional Notes
Ladder in white bands: 250kD, 150kD, 100kD, 75kD, 50kD, 37kD, etc. First two lanes are positive controls--untreated cell lysate. Third land is a lysate from cells that were treated with the adenovirus. I see a slight band at 108kD, a prominent band at 64kD, and another prominent band at 25kD, which I don't think is background, but another adenovirus protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 01 2008