Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (ab204695)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture media, Cell Lysate, Purified protein, Tissue Lysate
- Sensitivity: 10 µU
製品の概要
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製品名
Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)
ADA キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell Lysate, Cell culture media, Purified protein, Tissue Lysate -
アッセイタイプ
Enzyme activity -
検出感度
10 µU -
検出範囲
10 µU - 100 µU -
種交差性
交差種: Mammals, Other species -
製品の概要
Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (ab204695) is an assay where inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product that can be easily quantified at Ex/Em = 535/587 nm. The kit measures total activity of Adenosine Deaminase with limit of quantification of 10 μU recombinant Adenosine Deaminase. The range of the assay is from 10-100 μU.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K328 Adenosine Deaminase Activity Assay Kit (Fluorometric). K328-100 is the same size as the 100 test size of ab204695.
Adenosine Deaminase (ADA, EC 3.5.4.4) is an enzyme that catalyses the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and has been found associated with DPP4/CD26 in the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 10X ADA Assay Buffer 1 x 25ml ADA Convertor 1 vial Converter Enzyme VIII 1 vial ADA Positive Control 1 vial ADA Substrate 1 x 500µl Inosine Standard 1 x 100µl OxiRed Probe 1 x 200µl -
研究分野
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機能
Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. -
組織特異性
Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. -
関連疾患
Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency. -
配列類似性
Belongs to the adenosine and AMP deaminases family. -
細胞内局在
Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion. - Information by UniProt
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別名
- ada
- ADA_HUMAN
- ADA1
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画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab204695 は 6 報の論文で使用されています。
- Brown EM et al. Gut microbiome ADP-ribosyltransferases are widespread phage-encoded fitness factors. Cell Host Microbe 29:1351-1365.e11 (2021). PubMed: 34403684
- Sánchez-Melgar A et al. Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells. Front Neurosci 15:702817 (2021). PubMed: 34539333
- Zhang H et al. ADAR1 Facilitates KSHV Lytic Reactivation by Modulating the RLR-Dependent Signaling Pathway. Cell Rep 31:107564 (2020). PubMed: 32348766
- Sánchez-Melgar A et al. Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging. Int J Mol Sci 21:N/A (2020). PubMed: 33023260
- Sánchez-Melgar A et al. Resveratrol Modulates and Reverses the Age-Related Effect on Adenosine-Mediated Signalling in SAMP8 Mice. Mol Neurobiol 56:2881-2895 (2019). PubMed: 30069829
- Netsch P et al. Human mesenchymal stromal cells inhibit platelet activation and aggregation involving CD73-converted adenosine. Stem Cell Res Ther 9:184 (2018). PubMed: 29973267