製品の概要

  • 製品名
    Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric)
    ADA キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell Lysate, Purified protein, Tissue Lysate
  • アッセイタイプ
    Quantitative
  • 検出感度
    1000 µU
  • 種交差性
    交差種: Other species, Mammals
  • 製品の概要

    Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric) (ab204695) is an assay where inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA convertor and developer to generate uric acid that can be easily quantified at OD293 nm.


    The kit measures total activity of Adenosine Deaminase with limit of quantification of 1 mU recombinant Adenosine Deaminase.


     

  • 特記事項

    Adenosine Deaminase (ADA, EC 3.5.4.4) is an enzyme that catalyses the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and has been found associated with DPP4/CD26 in the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.

  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 100 tests
    ADA Assay Buffer (10x) 1 x 25ml
    ADA Convertor (30 U) 1 vial
    ADA Developer (10 U) 1 vial
    ADA Substrate 1 x 500µl
    ADA Positive Control (50 U) 1 vial
    Inosine Standard (10 mM) 1 x 100µl
    U.V. transparent plate (96-well) 1 unit
  • 研究分野
  • 機能
    Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
  • 組織特異性
    Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
  • 関連疾患
    Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
  • 配列類似性
    Belongs to the adenosine and AMP deaminases family.
  • 細胞内局在
    Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
  • Information by UniProt
  • 別名
    • ada
    • ADA_HUMAN
    • ADA1
    • Adenosine aminohydrolase
    • Adenosine deaminase
    see all

画像

  • Typical inosine standard calibration curve.

  • Kinetic curves showing ADA activity detection in positive control (included in the kit), Jurkat cell lysate (3 µg) and rat brain lysate (19 µg).

  • ADA specific activity in rat brain lysate (19 µg) and Jurkat cell lysate (3 µg).

プロトコール

参考文献

ab211093 has not yet been referenced specifically in any publications.

レビューと Q&A

Adenosine Deaminase Activity in C. elegans

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This product was tested in a set of mutation accumulation (MA) lines of Caenorhabditis elegans, as well as the N2 ancestor the MA lines were generated from. We were able to detect adenosine deaminase activity in all lines with a high degree of precision. There was a significant difference in the amount of adenosine deaminse activity between the lines, when total protein measure was corrected for. We intend to use this product to test for activity differences in a set of 43 MA lines as well as their ancestor.

Abcam user community

Verified customer

投稿 Feb 15 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録