Application
ELISA
Sample
Human Purified protein (human acetylcholinesterase from erythrocytes)
Specification
human acetylcholinesterase from erythrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Direct
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 0.1 M K2HPO4/KH2PO4, pH = 7.2
Secondary antibody
Name
Non-Abcam antibody was used: ANTI-GOAT IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Additional data
Additional Notes
0.01 % Tween20 in all incubation buffers (otherwise high amount of unspecific binding), 0.01 % Tween20 in washing buffer, detection of the antibodywas possible when coated (secondary antibody worked fine - also with other probes), antibody did not recognize the coated or otherwise fixated hAChE (fixated with polyclonal capture antibody or fasciculin 2), antibody also did not recognize other AChEs (mouse, torpedo)
anti-hAChE-ab also was used in more concentrated manner (highest concentration of about 2 µg/ml
anti-hAChE-ab also was used in more concentrated manner (highest concentration of about 2 µg/ml
Abcam response
Thanks for submitting and Abreview and for your honest feedback! This is the first report using this antibody in ELISA. Perhaps ab31276 is not effective for this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 29 2013