Application
Western blot
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide)
Loading amount
40 µg
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Pierce 31460
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Prime
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45, 25 kDa
Additional data
Additional Notes
Lane 1 and 2: 1:5,000 primary antibody
Lane 4 and 5: 1:10,000 primary antibody
Lane 7 and 8: secondary control (no primary antibody)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Shannon Cruzen
Verified customer
投稿 Sep 30 2014