Anti-ATP7b 抗体 (ab133731)
Key features and details
- Rabbit polyclonal to ATP7b
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATP7b antibody
ATP7b 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATP7b -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide from the N-Terminus of Human ATP7b.
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ポジティブ・コントロール
- Human lung (respiratory epithelium) tissue or HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 48% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. -
組織特異性
Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. -
関連疾患
Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
Contains 6 HMA domains. -
翻訳後修飾
Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form. -
細胞内局在
Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels. - Information by UniProt
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参照データベース
- Entrez Gene: 540 Human
- Entrez Gene: 11979 Mouse
- Entrez Gene: 24218 Rat
- Omim: 606882 Human
- SwissProt: P35670 Human
- SwissProt: Q64446 Mouse
- SwissProt: Q64535 Rat
- Unigene: 492280 Human
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別名
- ATP7B antibody
- ATP7B_HUMAN antibody
- ATPase, Cu(2+) transporting, beta polypeptide antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung (respiratory epithelium) tissue labelling ATP7b with ab133731 at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunofluorescence analysis of HeLa cells, labelling ATP7b with ab133731 at a 1/100 dilution (left image), or immunizing peptide (right image).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab133731 は 1 報の論文で使用されています。
- Tian H et al. Effects of Salvia miltiorrhiza extract on lung adenocarcinoma. Exp Ther Med 22:794 (2021). PubMed: 34093750