Anti-ATP5A 抗体 [7H10BD4F9] (ab110273)
Key features and details
- Mouse monoclonal [7H10BD4F9] to ATP5A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-ATP5A antibody [7H10BD4F9]
ATP5A 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7H10BD4F9] to ATP5A -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Full length protein corresponding to Cow ATP5A.
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ポジティブ・コントロール
- Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
Near homogeneity as judged by SDS-PAGE. ab110273 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
7H10BD4F9 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (6) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. -
組織特異性
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. -
配列類似性
Belongs to the ATPase alpha/beta chains family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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参照データベース
- Entrez Gene: 282578 Cow
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Entrez Gene: 65262 Rat
- Omim: 164360 Human
- SwissProt: P19483 Cow
- SwissProt: P25705 Human
- SwissProt: Q03265 Mouse
see all -
別名
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
see all
画像
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All lanes : Anti-ATP5A antibody [7H10BD4F9] (ab110273) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 60 kDa -
HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (76)
ab110273 は 76 報の論文で使用されています。
- Zhang Z et al. Ruthenium 360 and mitoxantrone inhibit mitochondrial calcium uniporter channel to prevent liver steatosis induced by high-fat diet. Br J Pharmacol 179:2678-2696 (2022). PubMed: 34862596
- Fernandez-Vizarra E & Zeviani M Blue-Native Electrophoresis to Study the OXPHOS Complexes. Methods Mol Biol 2192:287-311 (2021). PubMed: 33230780
- Zhang Z et al. Dietary calcium supplementation promotes the accumulation of intramuscular fat. J Anim Sci Biotechnol 12:94 (2021). PubMed: 34503581
- Leguina-Ruzzi A et al. Glucose-Induced Expression of DAPIT in Pancreatic ß-Cells. Biomolecules 10:N/A (2020). PubMed: 32664368
- Gómez-Caudillo L et al. Principal component analysis on LC-MS/MS and 2DE-MALDI-TOF in glioblastoma cell lines reveals that mitochondria act as organelle sensors of the metabolic state in glioblastoma. Oncol Rep 44:661-673 (2020). PubMed: 32468038