Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Human Cell lysate - nuclear (HeLa nuclear cell lysate)
Specification
HeLa nuclear cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% BSA in TBS-Tween20
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
BioRad Clarity ECL
Bands
Specific: 350 kDa
Exposure
10 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Nov 20 2013