Anti-ATIC 抗体 (ab186725)
Key features and details
- Rabbit polyclonal to ATIC
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATIC antibody
ATIC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATIC -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human ATIC.
Database link: P31939 -
ポジティブ・コントロール
- Extracts from MCF7, SW480, H460, HepG2, SW620, HT29 and K562 cell lines.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186725の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 65 kDa.
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IHC-P |
1/50 - 1/200.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 65 kDa. |
IHC-P
1/50 - 1/200. |
ターゲット情報
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機能
Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis. -
パスウェイ
Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1. -
関連疾患
Defects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness. -
配列類似性
Belongs to the purH family. -
ドメイン
The IMP cyclohydrolase activity resides in the N-terminal region. - Information by UniProt
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参照データベース
- Entrez Gene: 471 Human
- Omim: 601731 Human
- SwissProt: P31939 Human
- Unigene: 90280 Human
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別名
- AICAR formyltransferase/IMP cyclohydrolase antibody
- 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
- 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
see all
画像
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All lanes : Anti-ATIC antibody (ab186725) at 1/500 dilution
Lane 1 : Extract from MCF7 cell line.
Lane 2 : Extract from SW480 cell line.
Lane 3 : Extract from H460 cell line.
Lane 4 : Extract from HepG2 cell line.
Lane 5 : Extract from SW620 cell line.
Lane 6 : Extract from HT29 cell line.
Lane 7 : Extract from K562 cell line.
Predicted band size: 65 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling ATIC with ab186725 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling ATIC with ab186725 at 1/200. Magnification: 400x.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186725. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186725 は 1 報の論文で使用されています。
- Zhuang X et al. SHP-1 knockdown suppresses mitochondrial biogenesis and aggravates mitochondria-dependent apoptosis induced by all trans retinal through the STING/AMPK pathways. Mol Med 28:125 (2022). PubMed: 36273174