Anti-ATIC 抗体 [EPR13243-53] (ab188321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13243-53] to ATIC
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ATIC antibody [EPR13243-53]
ATIC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13243-53] to ATIC -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Jurkat, HeLa, HepG2 and HCT-116 cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13243-53 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab188321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/140.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
|
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IP |
1/40 - 1/60.
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特記事項 |
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Flow Cyt (Intra)
1/140. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
IP
1/40 - 1/60. |
ターゲット情報
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機能
Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis. -
パスウェイ
Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1. -
関連疾患
Defects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness. -
配列類似性
Belongs to the purH family. -
ドメイン
The IMP cyclohydrolase activity resides in the N-terminal region. - Information by UniProt
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参照データベース
- Entrez Gene: 471 Human
- Omim: 601731 Human
- SwissProt: P31939 Human
- Unigene: 90280 Human
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別名
- AICAR formyltransferase/IMP cyclohydrolase antibody
- 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
- 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
see all
画像
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All lanes : Anti-ATIC antibody [EPR13243-53] (ab188321) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HCT-116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab188321 は 1 報の論文で使用されています。
- Wang H et al. Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity. Cell Death Dis 10:627 (2019). PubMed: 31427568