Application
Western blot
Sample
Human Tissue lysate - whole (WIF-B cells, the hepatoma-derived hybrid cell line)
Gel Running Conditions
Reduced Denaturing (6% SDS-PAGE)
Loading amount
30 µg
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-Ms
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: between 55 and 72, a kDa Non-specific: around 95 and betwee kDa
Positive control
Specific band between 130 and 95 kDa most likely represents ER-localized ATF6, while the band between 72 and 55 (around 65 kDa) most likely represents ATF6 that has been trafficked to the Golgi and cleaved.
The bands above 130 kDa may represent dimer of ATF6.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
投稿 Dec 08 2016