Anti-ARSA/ASA 抗体 (ab74804)
Key features and details
- Rabbit polyclonal to ARSA/ASA
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARSA/ASA antibody
ARSA/ASA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ARSA/ASA -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human ARSA/ASA (internal sequence).
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ポジティブ・コントロール
- Human cervix carcinoma tissue. Jurkat and COLO 205 cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/1000. Predicted molecular weight: 54 kDa.
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特記事項 |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 54 kDa. |
ターゲット情報
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機能
Hydrolyzes cerebroside sulfate. -
関連疾患
Defects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
配列類似性
Belongs to the sulfatase family. -
翻訳後修飾
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 410 Human
- Entrez Gene: 11883 Mouse
- Entrez Gene: 315222 Rat
- Omim: 607574 Human
- SwissProt: P15289 Human
- SwissProt: P50428 Mouse
- Unigene: 88251 Human
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別名
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
see all
画像
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ab74804 at 1/50 dilution staining ARSA/ASA in human cervix carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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All lanes : Anti-ARSA/ASA antibody (ab74804) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : COLO 205 cell extracts
Lane 3 : Jurkat cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab74804 は論文での使用が確認できていません。