製品の概要

  • 製品名
    APC/Cy5.5® Conjugation Kit
  • 製品の概要

    APC/Cy5.5® Conjugation Kit ab102855 uses a simple and quick process to conjugate an antibody to HRP. It can also be used to conjugate other proteins or peptides.


    To conjugate an antibody to APC/Cy5.5® using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to APC/Cy5.5®.

  • 特記事項

    Amount and volume of antibody for conjugation to APC/Cy5.5®.

     Kit size   Recommended maximum
    amount of antibody
    Maximum antibody 
    volume1
    30 µg   3 x 10 µg 3 x 10 µL
    100 µg   100 µg  100 µL
    300 µg   3 x 100 µg  3 x 100 µL
    1 mg   1 mg 1 mL

    1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1%/1% BSA2 50% glycerol
    0.1% sodium azide PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.


    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  • アプリケーション
    適用あり: Conjugationmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Conjugation Use at an assay dependent dilution.

画像

  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

プロトコール

参考文献

ab102855 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry. We do have the the following APCCy5.5 conjugation kits available: ab102853 EasyLink APC/Cy5.5® Conjugation Kit (1x100 µg APC/Cy5.5®) Click here (or use the following: https://www.abcam.com/index.html?datasheet=102853).   ab1...

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This may be possible by performing a time course of conjugation. One could set up the conjugation and then remove small samples of the conjugate over the desired time course. The removed samples should be quenched immediately and then stored either on ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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