製品の概要

  • 製品名
    Anti-ALIX antibody [EPR15314] - N-terminal
    ALIX 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR15314] to ALIX - N-terminal
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment within Human ALIX aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8WUM4

  • ポジティブ・コントロール
    • 293, HeLa, K562, Jurkat, RAW 264.7, PC12, and NIH 3T3 cell lysates; Jurkat cells.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab186429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa).
Flow Cyt 1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
  • 配列類似性
    Contains 1 BRO1 domain.
  • 細胞内局在
    Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIP1 antibody
    • ALG 2 interacting protein 1 antibody
    • ALG-2-interacting protein 1 antibody
    • ALG2 interacting protein X antibody
    • Alix antibody
    • Apoptosis linked gene 2 interacting protein X antibody
    • Dopamine receptor interacting protein 4 antibody
    • DRIP4 antibody
    • Hp95 antibody
    • KIAA1375 antibody
    • MGC17003 antibody
    • PDC6I_HUMAN antibody
    • PDCD6 interacting protein antibody
    • PDCD6-interacting protein antibody
    • PDCD6IP antibody
    • Programmed cell death 6 interacting protein antibody
    • Programmed cell death 6-interacting protein antibody
    see all

画像

  • All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 246.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 97 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling ALIX with ab186429 at 1/240 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary at 1/150 dilution.

  • All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 97 kDa

参考文献

This product has been referenced in:
  • Pua HH  et al. Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses. Cell Rep 26:933-944.e4 (2019). Read more (PubMed: 30673615) »
  • Xie Z  et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29502100) »
See all 6 Publications for this product

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