The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204873 (AKT1 + PKN2 peptide substrate) can be utilized as a substrate for the following active protein kinases:
ab126914 (Active human CDC7 + DBF4 full length protein) ab70320 (Active human CDK9+CyclinK full length protein) ab60834 (Active human PDPK1 full length protein)
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Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
has not yet been referenced specifically in any publications.