Anti-ADCK3 抗体 (ab230897)
Key features and details
- Rabbit polyclonal to ADCK3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ADCK3 antibody -
製品の詳細
Rabbit polyclonal to ADCK3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Synthetic peptide corresponding to Human ADCK3 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q8NI60 -
ポジティブ・コントロール
- WB: HeLa and U-251 MG whole cell lysates. IHC-P: Human thymus tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab230897の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
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WB |
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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IHC-P
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
May be a chaperone-like protein essential for the proper conformation and functioning of protein complexes in the respiratory chain. -
組織特異性
Ubiquitously expressed with a relatively greater abundance in heart and skeletal muscle. -
関連疾患
Defects in ADCK3 are a cause of coenzyme Q10 deficiency (CoQ10 deficiency) [MIM:607426]. CoQ10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy.
Defects in ADCK3 are the cause of spinocerebellar ataxia autosomal recessive type 9 (SCAR9) [MIM:612016]; also known as autosomal recessive cerebellar ataxia type 2 (ARCA2). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR9 is an autosomal recessive form characterized by gait ataxia and cerebellar atrophy with slow progression and few associated features. Patients can manifest brisk tendon reflexes and Hoffmann sign, mild psychomotor retardation, mild axonal degeneration of the sural nerve, exercise intolerance and elevated serum lactate. -
配列類似性
Belongs to the protein kinase superfamily. ADCK protein kinase family.
Contains 1 protein kinase domain. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 536925 Cow
- Entrez Gene: 56997 Human
- Entrez Gene: 67426 Mouse
- Entrez Gene: 360887 Rat
- Omim: 606980 Human
- SwissProt: Q29RI0 Cow
- SwissProt: Q8NI60 Human
- SwissProt: Q60936 Mouse
see all -
別名
- aarF domain containing protein kinase 3 antibody
- aarF domain-containing protein kinase 3 antibody
- ADCK 3 antibody
see all
画像
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All lanes : Anti-ADCK3 antibody (ab230897) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Predicted band size: 72 kDa -
Formalin-fixed, paraffin-embedded human thymus tissue stained for ADCK3 using ab230897 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ADCK3 (red) using ab230897 at 1/100 dilution followed by DyLight 594-congugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab230897 は 1 報の論文で使用されています。
- Zhuo BB et al. ADCK1 is a potential therapeutic target of osteosarcoma. Cell Death Dis 13:954 (2022). PubMed: 36371387