Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen and oesophagus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen and oesophagus
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/6000
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
In e16 embryo, only smooth muscle gives strong positivity.
Submitted composite image shows this: upper image of oesophagus lower image of spleen/vein.
Abcam response
Thank you for your valuable feedback. Your data suggests that ab1997 may not be suitable to detect ADAM10 in mouse under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Sep 23 2015