Anti-ACTH 抗体 [AH26 + 57] - N-terminal (ab199007)
Key features and details
- Mouse monoclonal [AH26 + 57] to ACTH - N-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ACTH antibody [AH26 + 57] - N-terminal
ACTH 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AH26 + 57] to ACTH - N-terminal -
由来種
Mouse -
特異性
ab199007 is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide within Human ACTH aa 1 to the C-terminus (N terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. This sequence corresponds to amino acids 138-162 of the precursor POMC. (AH26)
Database link: P01189
Recombinant fragment (N terminal). (57)
Database link: P01189 -
ポジティブ・コントロール
- IHC-P: Human pituitary gland.
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特記事項
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
AH26 + 57 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199007の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 5443 Human
- Omim: 176830 Human
- SwissProt: P01189 Human
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別名
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
画像
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded human Pituitary Gland labeling ACTH with ab199007 at 2µg/mL (30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab199007 は 2 報の論文で使用されています。
- Ishii J et al. Endocrine secretory granule production is caused by a lack of REST and intragranular secretory content and accelerated by PROX1. J Mol Histol 53:437-448 (2022). PubMed: 35094211
- Zhang X et al. Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma. Elife 10:N/A (2021). PubMed: 34905486