Anti-ACADS/SCAD 抗体 [7E1AB5] (ab110318)
Key features and details
- Mouse monoclonal [7E1AB5] to ACADS/SCAD
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ACADS/SCAD antibody [7E1AB5]
ACADS/SCAD 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7E1AB5] to ACADS/SCAD -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Fibroblast, HeLa, HepG2. Human Heart FFPE tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
7E1AB5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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パスウェイ
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
関連疾患
Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. -
配列類似性
Belongs to the acyl-CoA dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 35 Human
- Omim: 606885 Human
- SwissProt: P16219 Human
- Unigene: 507076 Human
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別名
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
画像
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All lanes : Anti-ACADS/SCAD antibody [7E1AB5] (ab110318) at 1 µg/ml
Lane 1 : HepG2 whole cell cells at 10 µg
Lane 2 : ACADS at 0.01 µg
Predicted band size: 44 kDa -
Immunocytochemistry image of stained human fibroblasts. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110318 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
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IHC image of ACADS/SCAD staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110318, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab110318 は論文での使用が確認できていません。