Anti-ACAA2 抗体 (ab237540)
Key features and details
- Rabbit polyclonal to ACAA2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACAA2 antibody
ACAA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACAA2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Orangutan -
免疫原
Recombinant fragment corresponding to Human ACAA2 aa 150-300.
Database link: P42765 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity greater than 95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237540の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/1000.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
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ICC/IF |
1/50 - 1/500.
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特記事項 |
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IHC-P
1/500 - 1/1000. |
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa. |
ICC/IF
1/50 - 1/500. |
ターゲット情報
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機能
Abolishes BNIP3-mediated apoptosis and mitochondrial damage. -
パスウェイ
Lipid metabolism; fatty acid metabolism. -
配列類似性
Belongs to the thiolase family. -
細胞内局在
Mitochondrion. Colocalizes with BNIP3 in the mitochondria. - Information by UniProt
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参照データベース
- Entrez Gene: 10449 Human
- Entrez Gene: 52538 Mouse
- Entrez Gene: 100171644 Orangutan
- Entrez Gene: 170465 Rat
- Omim: 604770 Human
- SwissProt: P42765 Human
- SwissProt: Q8BWT1 Mouse
- SwissProt: Q5RES5 Orangutan
see all -
別名
- 3 ketoacyl CoA thiolase antibody
- 3 ketoacyl CoA thiolase mitochondrial antibody
- 3-ketoacyl-CoA thiolase, mitochondrial antibody
see all
画像
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All lanes : Anti-ACAA2 antibody (ab237540) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Rat lung lysate
Lane 6 : Mouse liver lysate
Lane 7 : Mouse lung lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa -
Paraffin-embedded human kidney tissue stained for ACAA2 using ab237540 at 1/700 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ACAA2 (green) using ab237540 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab237540 は 1 報の論文で使用されています。
- Feng Y et al. PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming. Int J Oncol 56:697-708 (2020). PubMed: 31922217