HRP Anti-ABCG1 抗体 [EP1366Y] (ab201776)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1366Y] to ABCG1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-ABCG1 antibody [EP1366Y]
ABCG1 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EP1366Y] to ABCG1 -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HUVEC whole cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1366Y -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa). |
ターゲット情報
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機能
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. -
組織特異性
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. -
配列類似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
細胞内局在
Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 510745 Cow
- Entrez Gene: 9619 Human
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- Unigene: 124649 Human
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別名
- ABC transporter 8 antibody
- ABC8 antibody
- ABCG1 antibody
see all
画像
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HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg with
HRP Anti-ABCG1 antibody [EP1366Y] (ab201776) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201776 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab201776 は 2 報の論文で使用されています。
- Chen M et al. Chemokine CCL20 promotes the paclitaxel resistance of CD44+CD117+ cells via the Notch1 signaling pathway in ovarian cancer. Mol Med Rep 24:N/A (2021). PubMed: 34278466
- Hang L et al. Ox-LDL Causes Endothelial Cell Injury Through ASK1/NLRP3-Mediated Inflammasome Activation via Endoplasmic Reticulum Stress. Drug Des Devel Ther 14:731-744 (2020). PubMed: 32158192