Anti-ABCC9 抗体 [N319A/14] - C-terminal (ab174629)
Key features and details
- Mouse monoclonal [N319A/14] to ABCC9 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-ABCC9 antibody [N319A/14] - C-terminal -
製品の詳細
Mouse monoclonal [N319A/14] to ABCC9 - C-terminal -
由来種
Mouse -
特異性
ab174629 does not crossreact with SUR2B. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit -
免疫原
Recombinant fragment within Mouse ABCC9 aa 1500 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P70170 -
ポジティブ・コントロール
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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特記事項
The clone number has been updated from S319A-14 to N319A/14, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N319A/14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174629の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 174 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 174 kDa. |
ターゲット情報
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機能
Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation. -
関連疾患
Defects in ABCC9 are the cause of cardiomyopathy dilated type 1O (CMD1O) [MIM:608569]; also known as dilated cardiomyopathy with ventricular tachycardia. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in ABCC9 are the cause of familial atrial fibrillation type 12 (ATFB12) [MIM:614050]. ATFB12 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. -
配列類似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10060 Human
- Entrez Gene: 20928 Mouse
- Entrez Gene: 100008700 Rabbit
- Entrez Gene: 25560 Rat
- Omim: 601439 Human
- SwissProt: O60706 Human
- SwissProt: P70170 Mouse
- SwissProt: P82451 Rabbit
see all -
別名
- ABC37 antibody
- abcC9 antibody
- ABCC9_HUMAN antibody
see all
画像
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Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629) at 1/1000 dilution + Rat brain membrane lysate at 10 µg
Secondary
Goat anti-mouse IgG at 1/200 dilution
Predicted band size: 174 kDa -
4% formaldehyde-fixed SK-N-BE cells labeling ABCC9 using ab174629 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab174629 は 1 報の論文で使用されています。
- D'Amore C et al. An NBD Derivative of the Selective Rat Toxicant Norbormide as a New Probe for Living Cell Imaging. Front Pharmacol 7:315 (2016). PubMed: 27721792