Anti-ABCB11/BSEP 抗体 (ab217532)
Key features and details
- Rabbit polyclonal to ABCB11/BSEP
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ABCB11/BSEP antibody
ABCB11/BSEP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ABCB11/BSEP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Mouse ABCB11/BSEP aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9QY30 -
ポジティブ・コントロール
- Rat pancreatic and human liver carcinoma tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab217532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500.
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特記事項 |
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IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. -
組織特異性
Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. -
関連疾患
Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. -
配列類似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
ドメイン
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8647 Human
- Entrez Gene: 27413 Mouse
- Entrez Gene: 83569 Rat
- Omim: 603201 Human
- SwissProt: O95342 Human
- SwissProt: Q9QY30 Mouse
- SwissProt: O70127 Rat
- Unigene: 658439 Human
see all -
別名
- ABC member 16, MDR/TAP subfamily antibody
- ABC16 antibody
- ABCB1 antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreatic tissue labeling ABCB11/BSEP with ab217532 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded human liver carcinoma tissue labeling ABCB11/BSEP with ab217532 at 1/500 dilution overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH 6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab217532 は 2 報の論文で使用されています。
- Chen YS et al. Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice. Cells 8:N/A (2019). PubMed: 30884843
- Lee S et al. Effects of Red Ginseng Extract on the Pharmacokinetics and Elimination of Methotrexate via Mrp2 Regulation. Molecules 23:N/A (2018). PubMed: 30424502