Anti-ABCB11/BSEP 抗体 (ab155421)
Key features and details
- Rabbit polyclonal to ABCB11/BSEP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ABCB11/BSEP antibody
ABCB11/BSEP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ABCB11/BSEP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human ABCB11/BSEP aa 474-745 (internal sequence).
Database link: O95342 -
ポジティブ・コントロール
- Jurkat whole cell lysate (ab7899), AGS xenograft, A431 cells.
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特記事項
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155421の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 146 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
|
特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 146 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. -
組織特異性
Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. -
関連疾患
Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. -
配列類似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
ドメイン
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8647 Human
- Omim: 603201 Human
- SwissProt: O95342 Human
- Unigene: 658439 Human
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別名
- ABC member 16, MDR/TAP subfamily antibody
- ABC16 antibody
- ABCB1 antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded AGS xenograft labeling ABCB11/BSEP with ab155421 antibody at 1/100.
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Anti-ABCB11/BSEP antibody (ab155421) at 1/1000 dilution + Jurkat whole cell
lysate at 30 µg
Predicted band size: 146 kDa
5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed A431 cells labeling ABCB11/BSEP with ab155421 at 1/500. Image at the right costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab155421 は 10 報の論文で使用されています。
- Liu M et al. Peptide modified geniposidic acid targets bone and effectively promotes osteogenesis. J Orthop Translat 38:23-31 (2023). PubMed: 36313979
- Wang W et al. Transcriptome and Gut Microbiota Profiling Analysis of ANIT-Induced Cholestasis and the Effects of Da-Huang-Xiao-Shi Decoction Intervention. Microbiol Spectr 10:e0324222 (2022). PubMed: 36409145
- Gao YD et al. [Cytotoxicity and underlying mechanism of evodiamine in HepG2 cells]. Beijing Da Xue Xue Bao Yi Xue Ban 53:1107-1114 (2021). PubMed: 34916690
- Liu L et al. Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver-Gut Axis. Front Pharmacol 11:12 (2020). PubMed: 32116693
- Wei X et al. Ethyl acetate extract of herpetospermum pedunculosum alleviates a-naphthylisothiocyanate-induced cholestasis by activating the farnesoid x receptor and suppressing oxidative stress and inflammation in rats. Phytomedicine 76:153257 (2020). PubMed: 32534360
- Yan Y et al. Phytochemicals protect L02 cells against hepatotoxicity induced by emodin via the Nrf2 signaling pathway. Toxicol Res (Camb) 8:1028-1034 (2019). PubMed: 32153769
- Naik PP et al. Autophagy regulates cisplatin-induced stemness and chemoresistance via the upregulation of CD44, ABCB1 and ADAM17 in oral squamous cell carcinoma. Cell Prolif 51:N/A (2018). PubMed: 29171106
- Gan L et al. Functional analysis of the correlation between ABCB11 gene mutation and primary intrahepatic stone. Mol Med Rep N/A:N/A (2018). PubMed: 30431138
- Mather Q et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). PubMed: 28781809
- Zhou JJ et al. Knockdown of long non-coding RNA HOTAIR sensitizes hepatocellular carcinoma cell to cisplatin by suppressing the STAT3/ABCB1 signaling pathway. Oncol Lett 14:7986-7992 (2017). PubMed: 29250186