7-Chlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist (ab120255)
Key features and details
- NMDA receptor glycine site antagonist; water soluble
- CAS Number: 1263094-00-3
- Purity: > 99%
Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
7-Chlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist -
製品の詳細
NMDA receptor glycine site antagonist; water soluble -
生理活性の詳細
Potent NMDA receptor glycine site antagonist. Water soluble form.
Also available in simple stock solutions (ab146692) - add 1 ml of water to get an exact, ready-to-use concentration. -
精製度
> 99% -
CAS 番号
1263094-00-3 -
構造式
製品の特性
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体系名
7-Chloro-4-hydroxyquinoline-2-carboxylic acid sodium salt
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分子量
245.60 -
PubChem 登録番号
52974249 -
保存方法
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
O=C(O[Na])c1cc(O)c2ccc(Cl)cc2n1 -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120255の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120255, 7-Chlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist
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ab12416 staining cGMP in SKNSH cells treated with 7-Chlorokynurenic acid sodium salt (ab120255), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 7-Chlorokynurenic acid sodium salt, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120255 (7-Chlorokynurenic acid sodium salt) in DMSO. Some samples where then further incubated with 1 mM glutamate (ab120049) for 15 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (3)
ab120255 は 3 報の論文で使用されています。
- Biane C et al. Developmental emergence of two-stage nonlinear synaptic integration in cerebellar interneurons. Elife 10:N/A (2021). PubMed: 34730085
- Raghunatha P et al. Microglial NMDA receptors drive pro-inflammatory responses via PARP-1/TRMP2 signaling. Glia 68:1421-1434 (2020). PubMed: 32036619
- Hansen KB et al. Implementation of a fluorescence-based screening assay identifies histamine H3 receptor antagonists clobenpropit and iodophenpropit as subunit-selective N-methyl-D-aspartate receptor antagonists. J Pharmacol Exp Ther 333:650-62 (2010). PubMed: 20197375