6X His tag® peptide (ab14943)

Submit a review Q&A (1)References (3)

Key features and details

  • Suitable for: Blocking

製品の詳細

関連製品

特性

Our Abpromise guarantee covers the use of ab14943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-6X His tag® antibody (ab14923)

  • 製品の状態

    Liquid

  • 備考

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名

    • 6 His epitope tag
    • Hexa His tag
    • HHHHHH epitope tag
    • HHHHHH tag
    • His tag
    • Polyhistidine Tag
    see all

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab14943 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14943 は 3 報の論文で使用されています。

  • Siddiqui MF  et al. Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32604729
  • Chen J  et al. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog 11:e1005053 (2015). PubMed: 26226614
  • Rani N  et al. FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis. Nat Commun 3:749 (2012). PubMed: 22434192

レビューと Q&A

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Question

I am interested in getting the - 6X His tag peptide (ab14943) or 6X His tag® peptide (ab14941). I would like to know the difference. Also, can you let me know whether the peptides contains (copper, nickel or cobalt) metal chelates?

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Answer

Ab14941 should have N terminal cys and ab14943 should have a C terminal cys.

Our peptides are supplied as lyophilised powder and re-suspended in TK buffer so contain the following:
30 mM HEPES,
150 mM NaCl,
2 mM EDTA,
0.001% tween 20,
pH 6.75

The EDTA in the buffer will chelate metal ions

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