Anti-6X His tag® 抗体 [RMH01] (ab219465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RMH01] to 6X His tag®
- Suitable for: ELISA, Sandwich ELISA, WB
- Reacts with: Species independent
製品の概要
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製品名
Anti-6X His tag® antibody [RMH01]
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Human monoclonal [RMH01] to 6X His tag® -
由来種
Human -
特異性
ab219465 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
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アプリケーション
適用あり: ELISA, Sandwich ELISA, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide (His-tag). (Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus).
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ポジティブ・コントロール
- WB: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (K36M) protein; Sandwich ELISA: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein; purified HisTag-PCSK9 recombinant protein
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特記事項
ab219465 is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (ab200537), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab219465 was purified from an animal origin–free culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
RMH01 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab219465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.2 µg/ml.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 0.2 µg/ml. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50µL/well of different concentrations of ab219465 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
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A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of ab219465 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody
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All lanes : Anti-6X His tag® antibody [RMH01] (ab219465) at 0.2 µg/ml
Lane 1 : 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Lane 2 : 293T cells untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Secondary
All lanes : HRP conjugated anti-Human IgG secondary antibody
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab219465 は 1 報の論文で使用されています。
- Luo S et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). PubMed: 29725470