Application
Western blot
Sample
Human Recombinant protein (protein produced in mammalian expression system)
Loading amount
1 µg
Specification
protein produced in mammalian expression system
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/2000
Incubation time
30 minute(s) · Temperature: 23°C · Diluent: added to BSA block solution
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 30+ kDa
Positive control
far right lane, known his tagged protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 29 2007