製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/30000.
ELISA 1/10000 - 1/100000.
ICC 1/200 - 1/500.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献

This product has been referenced in:
  • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). Read more (PubMed: 28604720) »
  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . Read more (PubMed: 27378283) »
See all 5 Publications for this product

レビューと Q&A

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1-3 of 3 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Mar 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Recombinant protein
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: PBS 10% FC
Type
Direct

Dr. Michael Aigner

Verified customer

投稿 Aug 15 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Sunil Verma

Verified customer

投稿 Aug 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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