製品の概要

  • 製品名

    Anti-6X His tag® antibody (HRP)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to 6X His tag® (HRP)
  • 由来種

    Goat
  • 標識

    HRP
  • アプリケーション

    適用あり: WB, ELISA, ICCmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/30000.
ELISA 1/10000 - 1/100000.
ICC 1/200 - 1/500.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献

This product has been referenced in:

  • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). Read more (PubMed: 28604720) »
  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . Read more (PubMed: 27378283) »
See all 5 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Mar 17 2010

Answer

I feel the best option would be for you to bind your mutant protein to the nickel plate. The ab1269 antibody can then be used to detect the his-tag on your mutant protein. It then leaves the HRP on the antibody free for detection. If you coat the plate with the ab1269 antibody, it will bind your mutant protein, but without a secondary antibody, you may have no means of detecting this.

Read More
Application
ELISA
Sample
Escherichia coli Recombinant protein
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: PBS 10% FC
Type
Direct

Dr. Michael Aigner

Verified customer

投稿 Aug 15 2005

Application
Western blot
Sample
Human Recombinant protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Sunil Verma

Verified customer

投稿 Aug 05 2005

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