Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Embryonic fibrobalst cells)
Total protein in input
1e+006 cells
Treatment
Influenza virus infection
Immuno-precipitation step
Other - Protein G - Dynabeads
Specification
Embryonic fibrobalst cells
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Home made serum raised against a His-tagged viral
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye® 680
Additional data
Additional Notes
Upon western blotting of the pull-down sample, it was observed that the viral protein of interest (which is not his-tagged) was pulled down non-specifically. However, His-tagged cellular protein of interest was precipitated efficiently. Since we were studying interaction between the cellular protein and viral protein, non-specific binding of the cellular-protein did not result in a conclusive result.
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投稿 Mar 25 2016