Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19 cells transfected with rRab27a-His)
Loading amount
20 µg
Specification
ARPE-19 cells transfected with rRab27a-His
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/Tween, 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 27 kDa
Negative control
Lysate of non transfected ARPE-19 cells
Additional data
Additional Notes
1. lysate of ARPE-19 cells transfected with empty pCDNA3 vector
2. lysate of ARPE-19 cells expressing Rab27a-His
2. lysate of ARPE-19 cells expressing Rab27a-His
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Dec 17 2008