Key features and details
- Mouse monoclonal [HIS.H8] to 6X His tag®
- Suitable for: ICC/IF, ELISA, Dot blot, WB, IP
- Reacts with: Species independent
- Isotype: IgG2b
製品名Anti-6X His tag® antibody [HIS.H8]
6X His tag® 一次抗体 製品一覧
製品の詳細Mouse monoclonal [HIS.H8] to 6X His tag®
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
ab18184 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus.
アプリケーション適用あり: ICC/IF, ELISA, Dot blot, WB, IPmore details
種交差性交差種: Species independent
6x His synthetic peptide.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Concentration information loading...
精製度Protein A purified
特記事項（精製）Purified from TCS by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab18184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.
Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer.
Lanes 6-9 : Purified PDCD5 from E. coli with ~60-70 ng protein.
The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).
Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.
All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution
Lane 1 : ARPE-19 whole cell lysate - non-transfected
Lane 2 : ARPE-19 whole cell lysate - transfected with rRab27a-His
Lysates/proteins at 20 µg per lane.
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
Blocked with 5% milk for 30 minutes at 25°C.
Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk.
LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg
Lane 2 : 6x His-tagged protein expressed in BL21 (pET15b) at 0.1 µg
All lanes : IRDye® 800CW goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 5 minutes
ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).
ab18184 は 169 報の論文で使用されています。
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