Anti-6X His tag® 抗体 [HIS.H8] (ab18184)
![Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.co.jp/ps/products/18/ab18184/Images/ab18184-26832-anti-6x-his-tag-antibody-hish8-immunoprecipitation.jpg "Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)")
Key features and details
- Mouse monoclonal [HIS.H8] to 6X His tag®
- Suitable for: ICC/IF, ELISA, Dot blot, WB, IP
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-6X His tag® antibody [HIS.H8]
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HIS.H8] to 6X His tag® -
由来種
Mouse -
特異性
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
ab18184 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus.
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アプリケーション
適用あり: ICC/IF, ELISA, Dot blot, WB, IPmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag®.
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ポジティブ･コントロール
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特記事項
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from TCS by Protein A affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
HIS.H8 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (3) |
1/200.
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| ELISA | (1) |
Use at an assay dependent concentration.
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| Dot blot |
Use at an assay dependent concentration.
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| WB | (21) |
1/1000.
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| IP | (5) |
1/200.
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| 特記事項 |
|---|
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ICC/IF
1/200. |
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ELISA
Use at an assay dependent concentration. |
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Dot blot
Use at an assay dependent concentration. |
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WB
1/1000. |
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IP
1/200. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Mr Chris Tracy
Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.
Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer.
Lanes 6-9 : Purified PDCD5 from E. coli with ~60-70 ng protein.
The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).
Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody. -
![Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.co.jp/ps/products/18/ab18184/Images/ab18184-261409-anti-6x-his-tag-antibody-hish8-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Vladimir MilenkovicAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution
Lane 1 : ARPE-19 whole cell lysate - non-transfected
Lane 2 : ARPE-19 whole cell lysate - transfected with rRab27a-His
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutesBlocked with 5% milk for 30 minutes at 25°C.
Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk.
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![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.co.jp/ps/products/18/ab18184/Images/ab18184-290288-anti-6x-his-tag-antibody-hish8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
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![Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.co.jp/ps/products/18/ab18184/Images/ab18184-261408-anti-6x-his-tag-antibody-hish8-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Rachel LohAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg
Lane 2 : 6x His-tagged protein expressed in BL21 (pET15b) at 0.1 µg
Secondary
All lanes : IRDye® 800CW goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.co.jp/ps/products/18/ab18184/Images/ab18184-26833-anti-6x-his-tag-antibody-hish8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an anonymous abreview.ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (208)
ab18184 は 208 報の論文で使用されています。
- Smith EM et al. The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction. Nucleic Acids Res 49:458-478 (2021). PubMed: 33332560
- Pasechnek A et al. Active Lysogeny in Listeria Monocytogenes Is a Bacteria-Phage Adaptive Response in the Mammalian Environment. Cell Rep 32:107956 (2020). PubMed: 32726621
- Zhu X et al. Molecular Characterization and Chemotactic Function of CXCL8 in Northeast Chinese Lamprey (Lethenteron morii). Front Immunol 11:1738 (2020). PubMed: 33013827
- Gardell JL et al. Human macrophages engineered to secrete a bispecific T cell engager support antigen-dependent T cell responses to glioblastoma. J Immunother Cancer 8:N/A (2020). PubMed: 33122397
- Nabeshima Y et al. Simple method for large-scale production of macrophage activating factor GcMAF. Sci Rep 10:19122 (2020). PubMed: 33154460