製品の概要

  • 製品名

    Anti-6X His tag® antibody (FITC)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to 6X His tag® (FITC)
  • 由来種

    Goat
  • 標識

    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション

    適用あり: ICCmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/200 - 1/2000.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献

This product has been referenced in:

  • Tai-Nagara I  et al. Placental labyrinth formation in mice requires endothelial FLRT2/UNC5B signaling. Development 144:2392-2401 (2017). Read more (PubMed: 28576770) »
See 1 Publication for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

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Answer

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the influence of fixation upon ability to detect tagged proteins.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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