製品の概要

  • 製品名

    Anti-6X His tag® antibody (Agarose)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to 6X His tag® (Agarose)
  • 由来種

    Goat
  • 標識

    Agarose
  • アプリケーション

    適用あり: IPmore details
    適用なし: ChIP
  • 種交差性

    交差種: Species independent
  • 免疫原

    Full length native

    HHHHHH

    (6-His)(purified): conjugated to KLH.

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.
  • 追加情報
    Is unsuitable for ChIP.
  • ターゲット情報

    • 関連性

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • 細胞内局在

      Depends upon the localization of the parent protein tagged with hexahistidine.
    • 別名

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:

    See 1 Publication for this product

    レビューと Q&A

    1-2 of 2 Abreviews or Q&A

    Answer

    Vielen Dank fürIhre Anfrage.

    Das Labor hat bestätig,dass in 400ul Puffer (50% Slurry)es 200ul Beads hat(und 100ugAntikörper).

    Hier ist ein Link zu den Beads, welchebenutzt wurden. Ich bin mir nicht sicher, wie Sie genau die Anzahl Partikel berechnen möchten/können. Bitte lassen Sie mich wissen, falls Sie mehr Informationen vom Labor bräuchten:...

    Ich hoffe, dies hilft Ihnen trotzdemweiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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    Question
    Answer

    The type of agarose beads that were used were - Uniflow 4 from Sterogene.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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