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Anti-6X His tag® 抗体 [AD1.1.10] (ab15149)

Submit a review Q&A (2)References (7)
Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149)

    Key features and details

    • Mouse monoclonal [AD1.1.10] to 6X His tag®
    • Suitable for: ICC/IF, IHC-Fr, IP, WB
    • Reacts with: Species independent
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 650 Europium HRP PE PerCP SureLight® APC

    製品の概要

    • 製品名

      Anti-6X His tag® antibody [AD1.1.10]
      6X His tag® 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [AD1.1.10] to 6X His tag®

    • 由来種

      Mouse

    • アプリケーション

      適用あり: ICC/IF, IHC-Fr, IP, WBmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Synthetic peptide corresponding to 6X His tag®. PAX6 transcription factor linked to histidine tag.

    • 特記事項

      The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.

      HIS-TAG® is a trademark of EMD Biosciences, Inc.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.20
      Preservative: 0.01% Sodium azide
      Constituents: 0.58% Sodium chloride, 95% Sodium phosphate
    • Concentration information loading...

    • 精製度

      Protein G purified

    • 特記事項（精製）

      Tissue cuture supernatant purified by Protein G affinity chromatography.

    • 一次抗体 備考

      ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

    • ポリ/モノ

      モノクローナル

    • クローン名

      AD1.1.10

    • ミエローマ

      NS1

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab15149の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    WB
    1/500 - 1/100.
    特記事項
    ICC/IF
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    WB
    1/500 - 1/100.

    ターゲット情報

    • 関連性

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.

    • 細胞内局在

      Depends upon the localization of the parent protein tagged with hexahistidine.

    • 別名

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    画像

    • Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149)
      Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149)
      All lanes : Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 0.5 µg/ml

      Lane 1 : 60 kDa 6X His tagged protein at 0.3 µg
      Lane 2 : 60 kDa 6X His tagged protein at 0.1 µg
      Lane 3 : 60 kDa 6X His tagged protein at 0.03 µg
      Lane 4 : 60 kDa 6X His tagged protein at 0.01 µg
      Lane 5 : 60 kDa 6X His tagged protein at 0.003 µg

      Secondary
      All lanes : Goat Anti-Mouse (HRP) at 0.04 µg/ml


      Imaged using West Pico chemiluminescent substrate.

    参考文献 (7)

    ab15149 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab15149 は 7 報の論文で使用されています。

    • Yi KS  et al. Broader neutralization of CT-P27 against influenza A subtypes by combining two human monoclonal antibodies. PLoS One 15:e0236172 (2020). PubMed: 32726321
    • Lam M  et al. Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress. Elife 9:N/A (2020). PubMed: 31904339
    • Moreland KT  et al. In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. PLoS One 9:e95333 (2014). WB . PubMed: 24748133
    • Smith MH  et al. Misfolded proteins induce aggregation of the lectin Yos9. J Biol Chem 289:25670-7 (2014). WB . PubMed: 25086047
    • Ma L  et al. Serum anti-CCNY autoantibody is an independent prognosis indicator for postoperative patients with early-stage nonsmall-cell lung carcinoma. Dis Markers 35:317-25 (2013). WB . PubMed: 24167380
    • Ma Z  et al. Targeting human epidermal growth factor receptor signaling with the neuregulin's heparin-binding domain. J Biol Chem 284:32108-15 (2009). ICC/IF . PubMed: 19717564
    • Bergman LM  et al. Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins. BMC Cell Biol 7:35 (2006). PubMed: 16999872

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    I was looking for an anti-His-tag antibody. I
    found that in the description in some of them (ab5000, ab18184) it is
    indicated that the antibody recognizes recombinant protein expressed
    in mammalian cells and in some other (ab15149, ab81663) it is
    specified that the antibody recognizes protein expressed in bacteria.
    If the antibody is raised against His-tag epitope can it recognize
    indifferently recombinant protein expressed either in bacteria either
    in mammalian or eukariotic cells? or not?
    I had some problem with an anti-His-tag antibody of another company
    since recognize the protein expressed in bacteria but not in mammalian
    in WB and I would like to understand the reason before to look for a
    product from a different company.

    Read More
    Answer

    I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

    Important aspects for his tag recognition are, the localisation of the tag (N- or C-terminal) and the length of the his tag, that should be identical with the antibody chosen.

    Furthermore it is important to confirm that the tagged protein is expressed in transfected or transformed cells. If the recombinant protein is expressed, it should not make a difference for the detection if either a bacteria or a mammalian system was used.

    However, some his tag antibodies could cross react with other proteins in the used system. For ab81663, ab15149 there may be seen some cross reactivity with extracts of insect or mammalian cells

    Read More

    Question

    1.does ab15149 recognize n terminal histidine tags ? 2. If so does require just 6x His or 6xHis plus something else at the n terminal for the recognition ?

    Read More
    Answer

    This antibody recognises proteins containing the motif HHHHHH (5x His) and it is therefore of value in detecting proteins containing histidine tags (either at the N- or C-terminal). The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

    Read More

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