Anti-6X His tag® 抗体 (ab14923)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-6X His tag® antibody
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 6X His tag® -
由来種
Rabbit -
特異性
ab14923 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus in transfected or transformed cells.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
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ポジティブ･コントロール
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein, ab2431.
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特記事項
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14923の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 µg/ml. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Western blot - Anti-6X His tag® antibody (ab14923)Anti-6X His tag® antibody (ab14923) at 0.6 µg/ml +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (14)
ab14923 は 14 報の論文で使用されています。
- Yang L et al. Interplay between REVEILLE1 and RGA-LIKE2 regulates seed dormancy and germination in Arabidopsis. New Phytol 225:1593-1605 (2020). PubMed: 31580487
- Salari S et al. Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice. Parasit Vectors 13:415 (2020). PubMed: 32787908
- Zhang X et al. SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis. Plant Commun 1:100080 (2020). PubMed: 33367258
- Yang L et al. PIF1 and RVE1 form a transcriptional feedback loop to control light-mediated seed germination in Arabidopsis. J Integr Plant Biol 62:1372-1384 (2020). PubMed: 32277734
- Metwally E et al. Calcium-Activated Calpain Specifically Cleaves Glutamate Receptor IIA But Not IIB at the Drosophila Neuromuscular Junction. J Neurosci 39:2776-2791 (2019). PubMed: 30705102
