Application
Western blot
Sample
Human Purified protein (Purified His-tagged extracellular human LDLR)
Loading amount
0.1 µg
Specification
Purified His-tagged extracellular human LDLR
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBS 0.1% Tween-20
Secondary antibody
Secondary antibody
None used
Detection
Detection method
SuperSignal (Pierce)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 140, 100 kDa
Positive control
Purified His-tagged protein (Lane 1-3)
Negative control
Purified untagged protein (Lane 4)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 25 2009