Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Hindbrain section)
Permeabilization
Yes - PBS + 0.1% Tween20
Specification
Hindbrain section
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.1%) + 3% BSA
Dilution
1/800
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor® 568 Rabbit Anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Regardless of the concentration used, this unfortunately did not work in the chick.
Abcam response
Thank you for sharing this data with us. We have not previously tested ab9035 on chick hindbrain sections and based on your abreview, this antibody does not work on chick, or in IHC-Fr, under these conditions. Abcam would welcome feedback from other customers currently using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 21 2014