Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: IHC
- Reacts with: Mouse, Rat, Cow
- Isotype: IgY
- 倫理基準に準拠 - アニマル・フリーの生産
製品名Anti-68kDa Neurofilament/NF-L antibody
68kDa Neurofilament/NF-L 一次抗体 製品一覧
製品の詳細Chicken polyclonal to 68kDa Neurofilament/NF-L
アプリケーション適用あり: IHCmore details
種交差性交差種: Mouse, Rat, Cow
Tissue, cells or virus corresponding to Cow 68kDa Neurofilament/NF-L.
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.
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保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
バッファーPreservative: 0.065% Sodium azide
Concentration information loading...
特記事項（精製）The IgY preparation was made by chloroform delipidation of egg yolk followed by polyethylene glycol precipitation.
一次抗体 備考To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.
The Abpromise guarantee
機能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
関連疾患Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
配列類似性Belongs to the intermediate filament family.
ドメインThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
All lanes : Anti-68kDa Neurofilament/NF-L antibody (ab24520) at 1/20000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse spinal cord lysate
Lane 5 : Cow spinal cord lysate
Predicted band size: 63 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed (transcardial perfusion) of free floating section of rat cerecellum tissue stained for 68kDa Neurofilament/NF-L (green) using ab24520 at 1/200 dilution. FOX3/NeuN is stained with an anti-FOX3/NeuN antibody (red).