Anti-68kDa Neurofilament/NF-L 抗体 (ab24520)
Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: IHC
- Reacts with: Mouse, Rat, Cow
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-68kDa Neurofilament/NF-L antibody
68kDa Neurofilament/NF-L 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to 68kDa Neurofilament/NF-L -
由来種
Chicken -
アプリケーション
適用あり: IHCmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Cow -
免疫原
Tissue, cells or virus corresponding to Cow 68kDa Neurofilament/NF-L.
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特記事項
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
特記事項（精製）
The IgY preparation was made by chloroform delipidation of egg yolk followed by polyethylene glycol precipitation. -
一次抗体 備考
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24520の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC |
1/2000.
|
| 特記事項 |
|---|
|
IHC
1/2000. |
ターゲット情報
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機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
関連疾患
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
配列類似性
Belongs to the intermediate filament family. -
ドメイン
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
翻訳後修飾
O-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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参照データベース
- Entrez Gene: 281348 Cow
- Entrez Gene: 18039 Mouse
- Entrez Gene: 83613 Rat
- SwissProt: P02548 Cow
- SwissProt: P08551 Mouse
- SwissProt: P19527 Rat
- Unigene: 1956 Mouse
- Unigene: 18568 Rat
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別名
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
see all
画像
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Western blot - Anti-68kDa Neurofilament/NF-L antibody (ab24520)All lanes : Anti-68kDa Neurofilament/NF-L antibody (ab24520) at 1/20000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse spinal cord lysate
Lane 5 : Cow spinal cord lysate
Predicted band size: 63 kDa -
Immunohistochemistry - Anti-68kDa Neurofilament/NF-L antibody (ab24520)Immunofluorescent analysis of 4% paraformaldehyde-fixed (transcardial perfusion) of free floating section of rat cerecellum tissue stained for 68kDa Neurofilament/NF-L (green) using ab24520 at 1/200 dilution. FOX3/NeuN is stained with an anti-FOX3/NeuN antibody (red).
データシートおよび資料
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Datasheet download
参考文献 (0)
ab24520 は論文での使用が確認できていません。
